PRODUCT INFORMATION Thermo Scientific CloneJET PCR Cloning Kit #K1231. Components of the kit were function tested in control experiment as described in the manual. Product Information: CloneJET PCR Cloning Kit. OF ANALYSIS All components of the kit were function tested in control experiment as described in the manual.

PRODUCT INFORMATION Thermo Scientific CloneJET PCR Cloning Kit #K1232 40 rxns Lot Expiry Date Store at -20 °C www.thermoscientific.com/onebio. CloneJET PCR Cloning Kit.pdf - Free download as PDF File (.pdf). All components of the kit were function tested in control experiment as described in the manual.

The pJET1.2/blunt cloning vector carries a lethal restriction enzyme gene that is disrupted by ligation of a DNA insert into the cloning site. As a result, only bacterial cells with recombinant plasmids are able to form colonies. If the pJET1.2/blunt vector re-circularizes without an insert, it expresses the lethal restriction enzyme which kills the host E.

Coli cell after transformation. This positive selection drastically accelerates the process of colony screening and eliminates additional costs required for blue/white selection. For convenience in mapping and manipulation of the insert, the pJET 1.2/blunt vector multiple cloning site contains two BgIII recognition sequences that flank the insertion site. In addition, the vector contains a T7 promoter for in vitro and in vivo transcription as well as sequencing of the insert. Blunt-end PCR products generated by proofreading DNA polymerases, such as Phusion DNA Polymerase, can be directly ligated into the vector in just 5 minutes. New Super Mario Bros Wii Wad File. PCR products with 3'-A overhangs are blunted prior to ligation in 5 minutes using the proprietary thermostable DNA blunting enzyme included in the CloneJet PCR Cloning Kit. The cloning efficiency of both blunt- and sticky-end fragments was comparable between the CloneJET cloning kit and the ligase-based cloning kit (Vendor A) as shown in Figure 1.